Repression of chilling-induced ACC accumulation in transgenic citrus by over-production of antisense 1-aminocyclopropane-1-carboxylate synthase RNA
Identifieur interne : 002E09 ( Main/Exploration ); précédent : 002E08; suivant : 002E10Repression of chilling-induced ACC accumulation in transgenic citrus by over-production of antisense 1-aminocyclopropane-1-carboxylate synthase RNA
Auteurs : WAI SHING WONG [Hong Kong] ; GENG GUANG LI [République populaire de Chine] ; WEN NING [Hong Kong] ; ZENG FU XU [République populaire de Chine] ; W. L. Wendy Hsiao [Hong Kong] ; LANG YIN ZHANG [République populaire de Chine] ; NING LI [Hong Kong]Source :
- Plant science : (Limerick) [ 0168-9452 ] ; 2001.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Plante transgénique.
English descriptors
- KwdEn :
Abstract
A chilling-inducible ACC synthase gene (CS-ACS1) has recently been identified from Citrus sinensis, This CS-ACS1 gene was constructed in an inverted orientation and placed under the control of the double 35S promoter. The antisense CS-ACS1 transgene was introduced into Carrizo citrange, C. sinensis (L.) Osbeck and Poncirus trifoliate by Agrobacterium-mediated gene transfer. The transformation efficiency in the transformation of citrus stem segment was improved significantly to 87 and 88% for Poncirus trifoliate (L.) Raf and Carrizo citrange, respectively. The transgenic citrus lines that produce higher level of antisense ACS RNA were found to repress the increase of ACC content following the chilling treatment. This work represents the first example in controlling the ethylene biosynthesis in citrus plants through the genetic engineering approach.
Affiliations:
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L. Wendy" last="Hsiao">W. L. Wendy Hsiao</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biology, The Hong Kong University of Science and Technology</s1><s2>Clear Water Bay</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Clear Water Bay</wicri:noRegion></affiliation></author><author><name sortKey="Lang Yin Zhang" sort="Lang Yin Zhang" uniqKey="Lang Yin Zhang" last="Lang Yin Zhang">LANG YIN ZHANG</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>The South China Institute of Botany</s1><s2>Guangzhou</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Ning Li" sort="Ning Li" uniqKey="Ning Li" last="Ning Li">NING LI</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biology, The Hong Kong University of Science and Technology</s1><s2>Clear Water Bay</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Clear Water Bay</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Plant science : (Limerick)</title><title level="j" type="abbreviated">Plant sci. : (Limerick)</title><idno type="ISSN">0168-9452</idno><imprint><date when="2001">2001</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Plant science : (Limerick)</title><title level="j" type="abbreviated">Plant sci. : (Limerick)</title><idno type="ISSN">0168-9452</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>1-Aminocyclopropane-1-carboxylate synthase</term><term>1-Aminocyclopropanecarboxylic acid</term><term>Biological accumulation</term><term>Biosynthesis</term><term>Citrus sinensis</term><term>Cold</term><term>Ethylene</term><term>Gene</term><term>Genetic transformation</term><term>Induction</term><term>Low temperature</term><term>Poncirus trifoliata</term><term>Transgenic plant</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Gène</term><term>1-Aminocyclopropane-1-carboxylate synthase</term><term>Induction</term><term>Froid</term><term>Transformation génétique</term><term>Plante transgénique</term><term>Accumulation biologique</term><term>Cyclopropanecarboxylique acide(1-amino)</term><term>Basse température</term><term>Citrus sinensis</term><term>Poncirus trifoliata</term><term>Ethylène</term><term>Biosynthèse</term><term>Citrus sinensis Poncirus trifoliata</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Plante transgénique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A chilling-inducible ACC synthase gene (CS-ACS1) has recently been identified from Citrus sinensis, This CS-ACS1 gene was constructed in an inverted orientation and placed under the control of the double 35S promoter. The antisense CS-ACS1 transgene was introduced into Carrizo citrange, C. sinensis (L.) Osbeck and Poncirus trifoliate by Agrobacterium-mediated gene transfer. The transformation efficiency in the transformation of citrus stem segment was improved significantly to 87 and 88% for Poncirus trifoliate (L.) Raf and Carrizo citrange, respectively. The transgenic citrus lines that produce higher level of antisense ACS RNA were found to repress the increase of ACC content following the chilling treatment. This work represents the first example in controlling the ethylene biosynthesis in citrus plants through the genetic engineering approach.</div></front></TEI><affiliations><list><country><li>Hong Kong</li><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><country name="Hong Kong"><noRegion><name sortKey="Wai Shing Wong" sort="Wai Shing Wong" uniqKey="Wai Shing Wong" last="Wai Shing Wong">WAI SHING WONG</name></noRegion><name sortKey="Hsiao, W L Wendy" sort="Hsiao, W L Wendy" uniqKey="Hsiao W" first="W. L. Wendy" last="Hsiao">W. L. Wendy Hsiao</name><name sortKey="Ning Li" sort="Ning Li" uniqKey="Ning Li" last="Ning Li">NING LI</name><name sortKey="Wen Ning" sort="Wen Ning" uniqKey="Wen Ning" last="Wen Ning">WEN NING</name></country><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Geng Guang Li" sort="Geng Guang Li" uniqKey="Geng Guang Li" last="Geng Guang Li">GENG GUANG LI</name></region><name sortKey="Lang Yin Zhang" sort="Lang Yin Zhang" uniqKey="Lang Yin Zhang" last="Lang Yin Zhang">LANG YIN ZHANG</name><name sortKey="Zeng Fu Xu" sort="Zeng Fu Xu" uniqKey="Zeng Fu Xu" last="Zeng Fu Xu">ZENG FU XU</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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